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Development of an In Vitro Method for the Evaluation of Candidate Repellents Against Leptotrombidium (Acari: Trombiculidae) Chiggers

Kriangkrai Lerdthusnee, Nittaya Khlaimanee, Taweesak Monkanna, Siriporn Mungviriya, Warisa Leepitakrat, Mustapha Debboun, Russell E. Coleman
DOI: http://dx.doi.org/10.1603/0022-2585-40.1.64 64-67 First published online: 1 January 2003


We developed a rapid and economical in vitro procedure with which to evaluate the efficacy of candidate repellents against chiggers. The procedure requires only 5 min and a small number of chiggers to obtain a valid estimate of the median effective dose. We used this procedure to evaluate the repellent activity of 11 compounds against the chigger, Leptotrombidium imphalum Vercammen-Grandjean and Langston. Median effective doses were determined for 10 of the 11 compounds. DM-165-2 (N,N-diethyl-3-flurobenzamide) was the only compound that was significantly more effective than deet.

  • Leptotrombidium
  • chiggers
  • scrub typhus
  • repellents
  • personal protection
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